Dualex葉綠素儀在番茄生理反應(yīng)的原位估計(jì)中的應(yīng)用

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

摘要 

本研究于2020年進(jìn)行，旨在研究褪黑素（10、50和100µM）在三種方法（浸泡、灌溉和葉面噴灑）下對暴露于根結(jié)線蟲的番茄幼苗的某些生理方面的影響。幼苗接種了1000株南方根結(jié)線蟲的感染性幼體[（Kofoid and White）Chitwood]。Dualex®光學(xué)傳感器用于原位測量總?cè)~綠素、黃酮醇和花青素含量以及氮平衡指數(shù)（NBI）。結(jié)果表明，雖然褪黑素對葉綠素含量沒有顯著影響，但褪黑素改善了南方根結(jié)線蟲對葉綠素的不利影響，這取決于濃度和方式。黃酮醇在灌溉植物中最高，在浸水植物中介于06-1.1之間，表現(xiàn)出顯著差異。應(yīng)用褪黑素的方法會影響NBI，浸泡會促進(jìn)NBI，而灌溉會導(dǎo)致顯著降低。與其他兩種方法相比，灌溉可顯著提高花青素含量。一個觀察結(jié)果是，在含有線蟲的土壤中施用中低濃度的褪黑素會增加花青素。褪黑素在對抗線蟲的反應(yīng)中具有價值，但需要進(jìn)一步闡明。

EFFECTS OF MELATONIN ON ROOT KNOT NEMATODE: IN SITU ESTIMAT ON OF PHYSIOLOGICAL RESPONSES IN TOMATO

Abstract

This research was conducted in 2020 to investigate the influence of melatonin (10, 50 and 100 µM) given in three methods (immersion, irrigation and foliar spraying), on some physiological aspects of tomato seedlings exposed to root knot nematodes. The seedlings were inoculated with 1000 infective juveniles of Meloidogyne incognita [(Kofoid and White) Chitwood]. Dualex ® optic sensor was used to in situ measure total chlorophylls, flavonols and anthocyanins contents and nitrogen balance index (NBI). Results indicated that while no significant effects were observed on chlorophyll content, melatonin ameliorated the adverse effects of M. incognita on chlorophyll depending on the concentration and mode. Flavonols were at the highest in the irrigated plants and the lowest in the immersed ones, between 06-1.1, exhibiting a significant difference. NBI was affected by the method the melatonin was applied, and immersing boosted it, while irrigation caused a significant decrease. Irrigation resulted in significantly higher anthocyanins compared to the other two methods. One observation was that applying melatonin in the low and medium concentrations to the nematode containing soil increased the anthocyanins. Melatonin merits a value in developing a response against the nematode but needs further elucidation.

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